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细胞检测
  • FC101-01.JPG
FC101-01.JPG

TransDetect® Cell  Counting Kit (CCK)


产品介绍

TransDetect® Cell Counting Kit,是一种基于水溶性四唑盐的细胞增殖和细胞毒性检测试剂盒。它在电子耦合试剂1-Methoxy PMS存在的情况下,可以被线粒体内的脱氢酶还原为可溶性的橙黄色甲臜(formazan)。甲臜的数量与活细胞的数量成正比。细胞增殖越快、细胞毒性越小、细胞数量越多,则颜色越深,颜色的深浅与细胞数量呈现良好的线性关系。该产品细胞毒性小,对后续实验没有影响,与MTTXTT,MTSWST-1相比,法检测灵敏度更高,线性范围更宽,适用于药物筛选,细胞增殖测定,细胞毒性测定和肿瘤药敏实验。   

产品组成

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References

Li P, Du J, Goodier JL, et al. Aicardi-Goutières syndrome protein TREX1 suppresses L1 and maintains genome integrity through exonuclease-independent ORF1p depletion.Nucleic Acids Res. 2017,45(8):4619-4631. 

Gu J , Wang D , Zhang J , et al. GFRα2 prompts cell growth and chemoresistance through down-regulating tumor suppressor gene PTEN via Mir-17-5p in pancreatic cancer[J]. Cancer Letters, 2016, 380(2):434-441.

Li J , Zhuang C , Liu Y , et al. Synthetic tetracycline-controllable shRNA targeting long non-coding RNA HOXD-AS1 inhibits the progression of bladder cancer[J]. Journal of Experimental & Clinical Cancer Research, 2016, 35(1):99.

Zhigang Zhu,a Jianliang Zhou,  Jingli Ding, et al. A novel approach to prepare a tissue engineering decellularized valve scaffold with poly(ethylene glycol)–poly(ε-caprolactone)[J]. RSC Adv. 2016, 6(17):14427-14438.

Min Q H , Wang X Z , Zhang J , et al. Exosomes derived from imatinib-resistant chronic myeloid leukemia cells mediate a horizontal transfer of drug-resistant trait by delivering miR-365[J]. Experimental Cell Research, 2017, 362(2):386-393.